3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid monosodium salt hydrate is a versatile chemical compound with applications in analytical chemistry. Its significance is evident in its utilization as a colorimetric indicator for ferroxidase assays, providing a reliable means for the quantitative determination of ferroxidase activity. Additionally, it serves as a reagent for the determination of various metal ions, including iron (Fe), cobalt (Co), silver (Ag), copper (Cu), and nickel (Ni), enabling precise analysis of metal concentrations in various samples. With its dual functionality as both a colorimetric indicator and a metal ion determination reagent, 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid monosodium salt hydrate emerges as a valuable tool in analytical chemistry, facilitating accurate and sensitive detection of metal ions in diverse samples.