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2-Pyridinol 1-oxide (HOPO) serves as a peptide coupling agent, acting as an alternative to HOBt. Its unique chemical properties enable it to form stable chelate complexes with transition metal ions. This compound finds application in various fields: 1. Fabrication of gold electrodes for electrochemical detection of europium using cyclic voltammetry. 2. Preparation of polymeric nickel (II) complexes of 2-hydroxypyridine-N-oxide. 3. Participation in the synthesis of 1-methoxypyrid-4-one, facilitated by sodium methoxide and methyl toluene-9-sulphonate. These applications underscore the versatility and utility of 2-Pyridinol 1-oxide in analytical chemistry, materials science, and organic synthesis.