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88321-09-9,MFCD00132883
Catalog No.:AA004899

88321-09-9 | E-64D

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1mg
98%
in stock  
$22.00   $15.00
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5mg
98%
in stock  
$53.00   $37.00
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10mg
98%
in stock  
$78.00   $55.00
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1g
98%
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$1,720.00   $1,204.00
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  • Technical Information
  • Properties
  • Literature
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  • Technical Information
  • Properties
  • Literature
Technical Information
Catalog Number:
AA004899
Chemical Name:
E-64D
CAS Number:
88321-09-9
Molecular Formula:
C17H30N2O5
Molecular Weight:
342.4305
MDL Number:
MFCD00132883
SMILES:
CCOC(=O)[C@H]1O[C@@H]1C(=O)N[C@H](C(=O)NCCC(C)C)CC(C)C
Properties
Properties
 
BP:
477.88°C (rough estimate)  
Form:
Solid  
MP:
126.2°C  
Refractive Index:
1.5800 (estimate)  
Solubility:
Soluble in DMSO, DMF or ethanol  
Storage:
Keep in dry area;-20 ℃;  

Computed Properties
 
Complexity:
450  
Covalently-Bonded Unit Count:
1  
Defined Atom Stereocenter Count:
3  
Heavy Atom Count:
24  
Hydrogen Bond Acceptor Count:
5  
Hydrogen Bond Donor Count:
2  
Rotatable Bond Count:
11  
XLogP3:
2.3  

Literature

Title: Squaric acid/4-aminoquinoline conjugates: novel potent antiplasmodial agents.

Journal: European journal of medicinal chemistry 20131101

Title: Exploration of natural compounds as sources of new bifunctional scaffolds targeting cholinesterases and beta amyloid aggregation: the case of chelerythrine.

Journal: Bioorganic & medicinal chemistry 20121115

Title: M-opsin protein degradation is inhibited by MG-132 in Rpe65⁻/⁻ retinal explant culture.

Journal: Molecular vision 20120101

Title: Isolation of reovirus T3D mutants capable of infecting human tumor cells independent of junction adhesion molecule-A.

Journal: PloS one 20120101

Title: Detecting autophagy in Arabidopsis roots by membrane-permeable cysteine protease inhibitor E-64d and endocytosis tracer FM4-64.

Journal: Plant signaling & behavior 20111201

Title: Rapamycin induces differentiation of glioma stem/progenitor cells by activating autophagy.

Journal: Chinese journal of cancer 20111001

Title: Malaria proteases mediate inside-out egress of gametocytes from red blood cells following parasite transmission to the mosquito.

Journal: Cellular microbiology 20110601

Title: Effects of photodynamic therapy on the endocytic pathway.

Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology 20110430

Title: The cysteine protease inhibitor, E64d, reduces brain amyloid-β and improves memory deficits in Alzheimer's disease animal models by inhibiting cathepsin B, but not BACE1, β-secretase activity.

Journal: Journal of Alzheimer's disease : JAD 20110101

Title: Regulation of cathepsin G reduces the activation of proinsulin-reactive T cells from type 1 diabetes patients.

Journal: PloS one 20110101

Title: Human and mouse perforin are processed in part through cleavage by the lysosomal cysteine proteinase cathepsin L.

Journal: Immunology 20101001

Title: Lysosomal membrane permeabilization and cathepsin release is a Bax/Bak-dependent, amplifying event of apoptosis in fibroblasts and monocytes.

Journal: Cell death and differentiation 20100701

Title: CCL2 and interleukin-6 promote survival of human CD11b+ peripheral blood mononuclear cells and induce M2-type macrophage polarization.

Journal: The Journal of biological chemistry 20091204

Title: Inhibition of calpain increases LIS1 expression and partially rescues in vivo phenotypes in a mouse model of lissencephaly.

Journal: Nature medicine 20091001

Title: Environmental effects on charge densities of biologically active molecules: do molecule crystal environments indeed approximate protein surroundings?

Journal: The journal of physical chemistry. B 20090416

Title: Superoxide-dependent cathepsin activation is associated with hypertensive myocardial remodeling and represents a target for angiotensin II type 1 receptor blocker treatment.

Journal: The American journal of pathology 20080801

Title: Inhibition of calpains improves memory and synaptic transmission in a mouse model of Alzheimer disease.

Journal: The Journal of clinical investigation 20080801

Title: Inhibitors of cathepsin B improve memory and reduce beta-amyloid in transgenic Alzheimer disease mice expressing the wild-type, but not the Swedish mutant, beta-secretase site of the amyloid precursor protein.

Journal: The Journal of biological chemistry 20080321

Title: [Inhibition of calpain expression by E-64d in the rat retina subjected to ischemia/reperfusion injury].

Journal: Molekuliarnaia biologiia 20080101

Title: Direct evidence for calpain involvement in apoptotic death of neurons in spinal cord injury in rats and neuroprotection with calpain inhibitor.

Journal: Neurochemical research 20071201

Title: Babesia bovis: effects of cysteine protease inhibitors on in vitro growth.

Journal: Experimental parasitology 20071001

Title: Inhibition of the activation of multiple serine proteases with a cathepsin C inhibitor requires sustained exposure to prevent pro-enzyme processing.

Journal: The Journal of biological chemistry 20070720

Title: Effect of a thiol proteinase inhibitor, E-64-d, on susceptibility to infection with Staphylococcus aureus in Chediak-Higashi syndrome (beige) mice.

Journal: International immunopharmacology 20070701

Title: ER stress (PERK/eIF2alpha phosphorylation) mediates the polyglutamine-induced LC3 conversion, an essential step for autophagy formation.

Journal: Cell death and differentiation 20070201

Title: Cysteine protease inhibitors block Toxoplasma gondii microneme secretion and cell invasion.

Journal: Antimicrobial agents and chemotherapy 20070201

Title: Cysteine protease inhibitors effectively reduce in vivo levels of brain beta-amyloid related to Alzheimer's disease.

Journal: Biological chemistry 20070201

Title: A novel type of autophagy occurs together with vacuole genesis in miniprotoplasts prepared from tobacco culture cells.

Journal: Autophagy 20070101

Title: Knockdown of NHERF1 enhances degradation of temperature rescued DeltaF508 CFTR from the cell surface of human airway cells.

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 20070101

Title: The oncolytic effect in vivo of reovirus on tumour cells that have survived reovirus cell killing in vitro.

Journal: British journal of cancer 20061023

Title: Neurovascular and neuronal protection by E64d after focal cerebral ischemia in rats.

Journal: Journal of neuroscience research 20060901

Title: Cathepsin and calpain inhibitor E64d attenuates matrix metalloproteinase-9 activity after focal cerebral ischemia in rats.

Journal: Stroke 20060701

Title: Identification and discrimination of extracellularly active cathepsins B and L in high-invasive melanoma cells.

Journal: Analytical biochemistry 20060601

Title: Zymogen activation in a reconstituted pancreatic acinar cell system.

Journal: American journal of physiology. Gastrointestinal and liver physiology 20060501

Title: Microsphere-based protease assays and screening application for lethal factor and factor Xa.

Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology 20060501

Title: Amelioration of experimental arthritis by a calpain-inhibitory compound: regulation of cytokine production by E-64-d in vivo and in vitro.

Journal: International immunology 20051001

Title: Inhibition of cysteine protease activity disturbs DNA replication and prevents mitosis in the early mitotic cell cycles of sea urchin embryos.

Journal: Journal of cellular physiology 20050801

Title: Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy.

Journal: Autophagy 20050701

Title: Cathepsins and BID are involved in the molecular switch between apoptosis and autophagy in breast cancer MCF-7 cells exposed to camptothecin.

Journal: Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 20050601

Title: Degradation of tyrosinase induced by phenylthiourea occurs following Golgi maturation.

Journal: Pigment cell research 20050401

Title: Endosomal proteases facilitate the fusion of endosomes with vacuoles at the final step of the endocytotic pathway.

Journal: The Plant journal : for cell and molecular biology 20050301

Title: Resistance of Entamoeba histolytica to the cysteine proteinase inhibitor E64 is associated with secretion of pro-enzymes and reduced pathogenicity.

Journal: The Journal of biological chemistry 20040910

Title: Human neuroblastoma (SH-SY5Y) cells are highly sensitive to the lysosomotropic aldehyde 3-aminopropanal.

Journal: Brain research 20040806

Title: uPARAP/endo180 directs lysosomal delivery and degradation of collagen IV.

Journal: Experimental cell research 20040201

Title: B cell receptor-mediated nuclear fragmentation proceeds in WEHI 231 cells in the absence of detectable DEVDase and FRase activity.

Journal: FEBS letters 20031009

Title: Calpain inhibitor prevented apoptosis and maintained transcription of proteolipid protein and myelin basic protein genes in rat spinal cord injury.

Journal: Journal of chemical neuroanatomy 20031001

Title: Protein kinase C inhibition induces DNA fragmentation in COLO 205 cells which is blocked by cysteine protease inhibition but not mediated through caspase-3.

Journal: Experimental cell research 20030910

Title: Alpha tonoplast intrinsic protein is specifically associated with vacuole membrane involved in an autophagic process.

Journal: Plant & cell physiology 20030801

Title: Development of an in vitro assay for the proteolytic processing of the CDP/Cux transcription factor.

Journal: Journal of biochemistry and molecular biology 20030731

Title: A 48-hour exposure of pancreatic islets to calpain inhibitors impairs mitochondrial fuel metabolism and the exocytosis of insulin.

Journal: Metabolism: clinical and experimental 20030501

Title: Calpain is activated in experimental uremia: is calpain a mediator of uremia-induced myocardial injury?

Journal: Kidney international 20030301

Title: A role for the protease falcipain 1 in host cell invasion by the human malaria parasite.

Journal: Science (New York, N.Y.) 20021206

Title: Effects of proteasome inhibitor (lactacystin) and cysteine protease inhibitor (E-64-d) on processes of mitosis in Xenopus embryonic cells.

Journal: Zoological science 20021101

Title: Involvement of lysosomal cysteine proteases in hydrogen peroxide-induced apoptosis in HL-60 cells.

Journal: Bioscience, biotechnology, and biochemistry 20020901

Title: Promotion of cathepsin L activity in newt spermatogonial apoptosis induced by prolactin.

Journal: FEBS letters 20020619

Title: Inhibition of calpain-mediated apoptosis by E-64 d-reduced immediate early gene (IEG) expression and reactive astrogliosis in the lesion and penumbra following spinal cord injury in rats.

Journal: Brain research 20011019

Title: Calpains play a role in insulin secretion and action.

Journal: Diabetes 20010901

Title: Rapid fusion of granules with neutrophil cell membranes in hypertensive patients may increase vascular damage.

Journal: American journal of hypertension 20010901

Title: A thiol proteinase inhibitor, E-64-d, corrects the abnormalities in concanavalin A cap formation and the lysosomal enzyme activity in leucocytes from patients with Chediak-Higashi syndrome by reversing the down-regulated protein kinase C activity.

Journal: Clinical and experimental immunology 20010801

Title: Cell death in spinal cord injury (SCI) requires de novo protein synthesis. Calpain inhibitor E-64-d provides neuroprotection in SCI lesion and penumbra.

Journal: Annals of the New York Academy of Sciences 20010601

Title: Caspase-3 activation by lysosomal enzymes in cytochrome c-independent apoptosis in myelodysplastic syndrome-derived cell line P39.

Journal: Cancer research 20010401

Title: Human osteoclast cathepsin K is processed intracellularly prior to attachment and bone resorption.

Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 20010301

Title: Activation of caspase-3 by lysosomal cysteine proteases and its role in 2,2'-azobis-(2-amidinopropane)dihydrochloride (AAPH)-induced apoptosis in HL-60 cells.

Journal: Journal of biochemistry 20010101

Title: Doh-Ura K, et al. Lysosomotropic agents and cysteine protease inhibitors inhibit scrapie-associated prion protein accumulation. J Virol. 2000 May;74(10):4894-7.

Title: Konjar S, et al. Human and mouse perforin are processed in part through cleavage by the lysosomal cysteine proteinase cathepsin L. Immunology. 2010 Oct;131(2):257-67.

Title: Mullins SR, et al. Three-dimensional cultures modeling premalignant progression of human breast epithelial cells: role of cysteine cathepsins. Biol Chem. 2012 Dec;393(12):1405-16.

Title: Hook G, et al. The cysteine protease inhibitor, E64d, reduces brain amyloid-β and improves memory deficits in Alzheimer's disease animal models by inhibiting cathepsin B, but not BACE1, β-secretase activity. J Alzheimers Dis. 2011;26(2):387-408.

Title: Cheng XW, et al. Superoxide-dependent cathepsin activation is associated with hypertensive myocardial remodeling and represents a target for angiotensin II type 1 receptor blocker treatment. Am J Pathol. 2008 Aug;173(2):358-69.

Title: Ji Yeun Kim, et al. Safe, High-Throughput Screening of Natural Compounds of MERS-CoV Entry Inhibitors Using a Pseudovirus Expressing MERS-CoV Spike Protein. Int J Antimicrob Agents. 2018 Nov;52(5):730-732.

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Tags:88321-09-9 Molecular Formula|88321-09-9 MDL|88321-09-9 SMILES|88321-09-9 E-64D